Cell Services

CRISPR Gene Knockout

E. coli CRISPR Gene Knockout
E. coli CRISPR
Gene Knockout
Optimized λ-Red system combined with CRISPR-Cas9 enables efficient gene deletion, applicable to metabolic engineering, resistance mechanism research, and synthetic biology.
Yeast CRISPR Gene Knockout
Yeast CRISPR
Gene Knockout
Applicable to Saccharomyces cerevisiae and Schizosaccharomyces pombe, supports single-gene and multi-gene knockout, combined with homology-directed repair (HDR) to enhance editing efficiency.
Human Cell CRISPR Gene Knockout
Human Cell CRISPR
Gene Knockout
Targeting HEK293, HeLa, HCT116, iPSC, and other mammalian cell lines, we provide end-to-end CRISPR gene editing services to efficiently, rapidly, and accurately achieve target gene editing. Suitable for functional gene screening, disease research, and drug target studies.
Gene Knockout Efficiency Verification
Gene Knockout Efficiency Verification
NucleoSequence provides full-process CRISPR gene editing technical services, including verification of target gene knockout efficiency (qPCR, Western blot, and gene sequencing), ensuring efficient and precise target gene knockout.
Multiplex Gene Editing
Multiplex Gene Editing
NucleoSequence's CRISPR gene editing service can meet the needs of simultaneous knockout of dual or multiple genes, enabling interaction from point to line to surface in gene research, helping scientific research get off to a winning start.
Stable Cell Line Construction
Stable Cell Line Construction
Using CRISPR technology, exogenous genes (fluorescent proteins, foreign genes) are stably integrated into the host cell genome. Screening and monoclonal culture are used to obtain cell lines that can long-term and stably express the target gene or protein.

CRISPR gene knockout technology utilizes CRISPR-Cas9-mediated gene editing to achieve highly efficient and specific target gene knockout, applicable to functional gene screening, regulatory mechanism research, cell and microbial model construction, and drug development. We offer CRISPR-Cas9 gene knockout services for E. coli, yeast, and human cells, ensuring efficient gene editing and accurate validation.

CRISPR gene knockout technical services provide high-efficiency, low-off-target gene editing solutions widely applied in microbial genetic manipulation, functional genomics, disease model construction, and gene therapy research, promoting precision medicine and biotechnology advancement.